http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113670869-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_653c418e41edbb4e1ce8bc5e53e70cec |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6417 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6441 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6432 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-42 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
filingDate | 2021-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4fe524e959a8a69c87a4d03abcbb6d19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a616f0763ff34c4808bd0cffcce02a42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5f002612c4c0d7e7047817916d7c722d |
publicationDate | 2021-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-113670869-A |
titleOfInvention | A kind of biosensor for detecting alkaline phosphatase, its detection method and application |
abstract | The invention relates to the technical field of detection and analysis, in particular to a biosensor for detecting alkaline phosphatase, a detection method and application thereof, the sensor comprises a DNA probe, terminal deoxynucleotidyl transferase TdT and 2'-deoxythymidine ‑5'‑Triphosphate dTTPs. The biosensor and detection method of the present invention have good specificity and high sensitivity, due to the high specificity of ALP-catalyzed dephosphorylation and TdT-mediated template-free polymerization, 3' end repair-driven poly-A MBs dendritic self- The assembly has a high amplification efficiency, and the template-free TdT-assisted extension, assembly and activation of poly-A MBs is very easy and can be quickly achieved in the next step under isothermal conditions, requiring only one DNA probe (i.e. poly-A MB) , and the extension, assembly and activation of poly-A MB can be completed with only one tool enzyme (i.e. template-independent TdT); kinetic parameters can be evaluated, potential inhibitors can be screened, cytostatic effects can be estimated, and even human serum can be measured Alkaline phosphatase in samples for clinical trials. |
priorityDate | 2021-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 74.