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filingDate 2021-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_882568504b6419c180242dc714910c5d
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publicationDate 2021-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-113658636-A
titleOfInvention Method for evaluating quality of pluripotent stem cells
abstract The invention discloses a method for evaluating the quality of pluripotent stem cells, which comprises the following steps: performing sequence determination on the cells to be detected to obtain transcriptome expression lineage data and whole genome sequence data; performing statistics of the expression amount of the gene based on the transcriptome expression lineage data to obtain a mass fraction 1 of the cell; performing whole genome DNA variation analysis based on whole genome sequencing data; the cell mass fraction 2 was obtained from the obtained mass fraction of cells and the results of the whole genome DNA mutation analysis, and the pluripotent stem cell mass was evaluated. The method of the invention evaluates the cell quality by integrating multiple aspects, reduces a large amount of experimental processes by adopting a sequencing means, evaluates the quality of the pluripotent stem cells only from the level of transcriptome sequencing and whole genome sequencing, quickly shortens the time, and obtains reliable and accurate data results through statistical processing.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023134390-A1
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priorityDate 2021-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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