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filingDate 2021-08-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2021-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-113655222-A
titleOfInvention Method for researching micro distribution of hemicellulose in plant cell wall
abstract The invention belongs to the technical field of plant ultrastructure detection, and particularly relates to a method for researching micro-distribution of hemicellulose in plant cell walls. Firstly, carrying out wax removal, ash removal and lignin removal on a plant tissue slice, and then carrying out barium hydroxide selective fractional extraction on the plant tissue slice to separate hemicellulose; then, washing and sealing the plant tissue slices at different extraction stages and incubating the slices with a first antibody; washing the plant tissue section after the first antibody incubation and performing second antibody incubation; and finally observing the plant tissue section by using a laser confocal microscope. According to the invention, the hemicellulose is selectively extracted by barium hydroxide in a grading manner and the first antibody capable of specifically binding different types of hemicellulose is combined, so that the distribution of different types of hemicellulose in plant cell walls can be effectively and accurately identified and observed.
priorityDate 2021-08-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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