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filingDate 2021-09-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2021-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-113652388-A
titleOfInvention A kind of high-purity exosome extraction kit and extraction method thereof
abstract The invention discloses a high-purity exosome extraction kit and an extraction method thereof, belonging to the technical field of exosome separation, and an extraction method of a high-purity exosome extraction kit, including sample preparation, protein A agarose purification Bead preparation and affinity capture, this program also provides a high-purity exosome extraction kit, including a box body, a box cover, a collection tube, a filter, and a reagent bottle loaded in the box body, and several reagent bottles are encapsulated with protein A Sepharose purification beads, antibody mixture, washing solution, eluent, blood coagulant and PBS buffer, this protocol uses immunoaffinity capture method to obtain exosomes in blood/body fluids, which can be used for exosomes from different sources. The high-purity extraction of exosomes provides a stable and effective solution. The obtained exosomes have high purity and can be used for downstream analysis and liquid biopsy marker screening that require high exosome purity, such as RNASeq and mass spectrometry detection.
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