| abstract |
The invention discloses a cell climbing sheet for detecting acetylcholine receptor autoantibodies in human body fluids, a preparation method and application thereof, and belongs to the field of biotechnology. The adopted scheme includes: co-transfecting each subunit of the acetylcholine receptor and the empty vector plasmid according to a certain ratio, and then fixing, washing, terminating, washing and drying to prepare the detection of the acetylcholine receptor itself in human fluid. Antibody slides. Compared with the radioimmunoassay and the enzyme-linked immunosorbent assay, the method has higher sensitivity; compared with the immunofluorescence method reported in the literature of the prior art, the detection specificity is improved while the background is reduced. Therefore, the present invention has a good application prospect no matter from the research point of view or as an auxiliary diagnostic method for myasthenia gravis diseases. |