http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113637798-A

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filingDate 2021-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5a82c91c832c09fe72989145c949ffd8
publicationDate 2021-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-113637798-A
titleOfInvention Primers, probes and applications for detecting the deletion mutation site of S gene Δ69/70HV of 2019-nCoV Alpha strain
abstract The invention discloses primers, probes and applications for detecting the Δ69/70HV deletion mutation site of the new coronavirus Alpha strain S gene, and belongs to the field of biotechnology. The invention optimizes the primer sequence and fluorescent probe sequence of the Δ69/70HV deletion mutation site on the S gene in the Alpha mutant strain, the optimized sequence has the advantages of high specificity, sensitive signal, etc., and can effectively pass the Δ69/70del primer. , Probe to quickly detect whether the new coronavirus strain to be tested has the S gene deleted by Δ69/70HV, which can be used as one of the effective proofs to determine whether it is an Alpha strain, and to determine whether the new coronavirus strain to be tested is an Alpha mutant strain. testing method. After adding the S gene amplification primers and fluorescent probe mixtures of the new coronavirus Alpha strain of the present invention and the original strain at the same time into the reaction tube, there is no need to set up two reaction tubes for PCR detection respectively, and the same reaction tube can be used for PCR detection. It can be identified whether the sample to be tested is the S gene of the original strain or the S gene Δ69/70HV deletion type of the Alpha strain.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113913406-A
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