| abstract |
The invention provides a visual nucleic acid detection method combining isothermal amplification technology with exonuclease mediation, which is characterized in that the isothermal amplification technology is utilized to amplify nucleic acid to be detected, the 3 'terminal sequence of an Exo III shearing amplicon exposes a single-stranded DNA target, a stem-loop probe is combined with the target, the Exo III can shear the 3' terminal of the probe and release a fluorescent group, and virus nucleic acid can be quickly and sensitively detected by naked eyes or a transverse flow test strip. The method does not need a special detection device, has high detection speed, high sensitivity and low cost, can complete detection at room temperature, completes the naked eye and test strip visualization process within 25-30 min, and has detection sensitivity reaching 2 copies. |