| abstract |
The invention discloses a grifolan glucosyltransferase gfgel4, and a coding gene and application thereof, belongs to the technical field of edible fungus heredity and genetic engineering, and particularly relates to grifolan glucosyltransferase gfgel4, and a coding gene and application thereof; the invention clones the coding gene of the key enzyme beta-1, 3 glucosyltransferase for forming the branch structure in the glucosan synthesis process from the grifola frondosa mycelium genome for the first time, and proves the key role of the gene in the growth of the grifola frondosa mycelium, the glucosan synthesis and the formation of the glucosan branch structure through gene silencing; finally, beta-1, 3 glucosyltransferase is overexpressed in the grifola frondosa by a genetic engineering technology, so that the glucan synthesis yield is improved, and the branching degree of glucan is improved; the invention is helpful to understand the synthesis mechanism of edible and medicinal fungi polysaccharide from molecular level, and provides technical scheme and guidance for improving glucan synthesis path of edible and medicinal fungi by using genetic engineering technology. |