| abstract |
The invention relates to a base editing tool and a construction method thereof. In order to improve the efficiency and accuracy of target base editing in the existing PE2 system, the present invention provides a new base editing tool and its construction method. The base editing tool described in the base editing tool includes SunTag system and PE2 system. The present invention utilizes the results of the SunTag system and the PE system for the first time, adopts the SunTag system and the PE2 version with higher editing efficiency, and constructs the SunTag-PE2 system. Editing accuracy, enhancing editing accuracy, and providing more choices for gene editing, this technology will promote the rapid development of life science research, agricultural production, and biomedicine in my country and the world, and has important application value. |