| abstract |
The present invention relates to a base editing tool and a construction method thereof. In order to improve the target base editing efficiency and accuracy of the existing PE2 system, the present invention provides a new base editing tool and a construction method thereof. The base editing tool includes the SunTag system and PE2 system. The invention utilizes the results of the SunTag system and the PE system for the first time, adopts the SunTag system and the PE2 version with higher editing efficiency, and constructs the SunTag-PE2 system. After optimization, the HEK293T cells are compared with the original PE2 system, which can further improve the PE2 system. Editing accuracy, enhancing editing accuracy, and providing more options for gene editing, this technology will promote the rapid development of life science research, agricultural production and biomedicine in my country and the world, and has important application value. |