http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113633825-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61L2300-414 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61L27-3604 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61L27-227 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61L27-3691 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61L27-3641 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61L27-3687 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61L27-54 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L27-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L27-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L27-54 |
filingDate | 2021-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-113633825-B |
titleOfInvention | A kind of preparation method and application of heparinized decellularized fat material loaded with bFGF |
abstract | The invention provides a preparation method and application of a bFGF-loaded heparinized decellularized fat material. The bFGF-loaded heparinized DAT is obtained by freezing and thawing freshly sourced fat at a temperature of -80°C to 37°C, then placing it in Trypsin-EDTA digestion solution for shaking digestion, washing and then placing it in isopropanol. After washing with shaking and immersion, shaking and digesting with Triton X-100 detergent, shaking with PBS solution containing Benzonase, shaking with isopropanol, sterilizing and sterilizing, adding MES buffer containing EDC, NHS and heparin sodium. Heparinized in a liquid mixture solution, soaked in sterile bFGF solution to obtain bFGF-loaded heparinized decellularized adipose material, injected into a 3D printed scaffold made of natural polymers, and embedded in the body together as a fat filler It is used for regeneration or reconstruction of adipose tissue and induces adipogenesis in living body, and has good application effect. |
priorityDate | 2021-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 77.