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filingDate 2021-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_90972a733c2b2076c335a55144fd1ca6
publicationDate 2021-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-113633568-A
titleOfInvention A kind of preparation method of incomplete enzyme cut sericin anti-oxidation and anti-aging stock solution
abstract The invention discloses a method for preparing an anti-oxidative and anti-primitive stock solution of incompletely enzymatically cut sericin, comprising the following steps: first, 100 g of sericin powder is dissolved in 1 L of distilled water to prepare A solution with a concentration of 0.1 g/ml ; Dissolve 100g of protease in 1L of distilled water to prepare a B solution with a pH of 7.0 and a concentration of 0.1g/ml; 10g of trehalose powder and 10g of hyaluronic acid are dissolved in 1L of distilled water according to a 1:1 ratio to prepare a concentration of 0.01g /ml of C solution; heat A solution to 50 degrees, add B solution according to the ratio of 1/100 of A solution (the ratio of enzyme and protein is 1:100) to maintain the temperature, and test the pH value of 7, such as conditions Change and use NAHO to adjust the pH value to maintain at 7; after two hours of reaction, heat the solution to 80-100 degrees to destroy the protease, terminate the hydrolysis reaction of the enzyme, and add 1/10 of the C solution to stabilize after cooling to room temperature. The invention can maintain the stability of the incompletely hydrolyzed protein components, so that the optimal free radical scavenging stable state can be achieved.
priorityDate 2021-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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