http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113621624-A

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filingDate 2019-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f47b551d6987b955d70db321cfca554f
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publicationDate 2021-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-113621624-A
titleOfInvention Application of nucleotide sequence
abstract The invention provides application of a nucleotide sequence, belongs to the technical field of crop breeding, and can solve the technical problems of unsatisfactory fruit setting rate under adverse conditions such as high temperature and low temperature and the like in the conventional method for cultivating a tomato parthenocarpic line. The technical scheme comprises the following steps: the application of a group of nucleotide sequences in cultivating autonomous cross-pollination autonomous parthenocarpic lines of tomatoes comprises the following nucleotide sequences: the nucleotide sequence shown as SEQ ID NO.6 and SEQ ID NO.2 or the nucleotide sequence shown as SEQ ID NO.7 and SEQ ID NO. 8; the tomato self-cross pollination self-parthenocarpy line is obtained by cultivating the following method, and specifically comprises the following steps: selecting a plurality of target sites from a tomato genome, and constructing a vector for knocking out the target sites; and introducing the constructed vector into a tomato explant to perform tissue culture to obtain a transformed seedling, screening out a positive strain from the transformed seedling, and identifying to obtain the autonomous cross pollination autonomous parthenocarpic line of the tomato.
priorityDate 2019-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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