| abstract |
The invention discloses a litchi-shaped nano-zinc oxide cluster adjuvant, a preparation method thereof, and an application as a vaccine adjuvant. The method comprises: adding a zinc salt into a polyol solvent and mixing to obtain a zinc salt polyol solution; The zinc salt polyol solution is first heated, then deionized water is added, and the second heating is performed until the solution becomes turbid, and the heat source is removed and the reaction product is obtained by natural cooling; wherein, the deionized water and the zinc salt The volume ratio of the polyol solution is 1:15-100; the reaction product is subjected to solid-liquid separation to obtain zinc oxide nano-cluster precipitation, followed by washing to obtain litchi-shaped nano-zinc oxide clusters. The surface of the litchi-shaped nano-zinc oxide cluster adjuvant is uneven, which can effectively improve the adsorption capacity of the antigen; the zinc oxide nano-cluster is similar to the particles of viruses and bacteria, which can increase the recognition and phagocytosis of immune cells and improve the efficiency of antigen presentation. |