| abstract |
The problem of the present disclosure is to provide an antigen-binding molecule, a polynucleotide encoding the antigen-binding molecule, a vector comprising the polynucleotide, a cell retaining the vector, a library comprising a plurality of the antigen-binding molecules that are each different, the antigen-binding molecule comprising the antigen A pharmaceutical composition of a binding molecule, a method of screening the antigen-binding molecule, a method of preparing the same, and the like, wherein the antigen-binding activity of the antigen-binding molecule varies according to the concentration of a target tissue-specific low-molecular-weight compound. The present inventors discovered methylthioadenosine (MTA) as a tumor tissue-specific low-molecular-weight compound, and created an antigen-binding domain whose binding activity to an antigen varies depending on the concentration of MTA, or an antigen-binding domain comprising the same. For antigen-binding molecules, a library containing a plurality of antigen-binding domains that are different from each other or antigen-binding molecules including the antigen-binding domains has been created, and it has been found that the above-mentioned problems can be solved by applying the library. By applying the antigen-binding molecules of the present disclosure, various diseases (eg, cancer) caused by the target tissue (eg, tumor tissue) can be specifically treated by the target tissue. |