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filingDate 2021-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5a4db8e05a4793ced500693604eb2a0d
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publicationDate 2021-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-113604521-A
titleOfInvention Method for enhancing yield of N-acetyl-5-hydroxytryptamine
abstract The invention relates to a method for enhancing the yield of N-acetyl-5-hydroxytryptamine. Converting a substrate 5-hydroxytryptamine by a recombinant cell containing a functional gene related to catalyzing 5-hydroxytryptamine to generate N-acetyl-5-hydroxytryptamine to obtain N-acetyl-5-hydroxytryptamine, and expressing an N-acetyl-5-hydroxytryptamine transferase gene or an acetyl coenzyme A synthetase gene for enhancing the yield of N-acetyl-5-hydroxytryptamine by the recombinant cell containing the functional gene related to catalyzing 5-hydroxytryptamine to generate N-acetyl-5-hydroxytryptamine, so as to produce N-acetyl-5-hydroxytryptamine transferase and acetyl coenzyme A synthetase. According to the invention, through overexpression of the AANTA gene and the ACS gene, the generation of N-acetyl-5-hydroxytryptamine from 5-hydroxytryptamine serving as an AANAT catalytic substrate is enhanced, and the synthetic yield of the N-acetyl-5-hydroxytryptamine is obviously improved.
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