| abstract |
The invention relates to the technical field of microbial fermentation, and discloses the application of recombinant Bacillus subtilis and a method for producing tetrahydropyrimidine by using waste water from synthesizing N-acetylneuraminic acid by an enzymatic method. In the present invention, a recombinant Bacillus subtilis is constructed through genetic engineering technology, which can ferment the waste water from the enzymatic synthesis of N-acetylneuraminic acid, and obtain a fermentation liquid containing tetrahydropyrimidine. After extraction, not only more tetrahydropyrimidines can be obtained, but also by-product bacterial residue protein feed can be obtained, and the discharged wastewater is 70.6-96.4% lower than the COD, BOD, total phosphorus, ammonia nitrogen, etc. in the original wastewater, and the method is also easy to operate. , saving resources, safety and environmental protection, low cost and other advantages, suitable for industrial application. |