| abstract |
The invention relates to a single molecule array detection (Simoa) kit of a biomarker CEA in an immunodetection technology and a using method thereof. The Simoa kit is used for detecting the content of carcinoembryonic antigen (CEA) protein in human body fluid based on a Simoa platform monomolecular array technology and a double-antibody sandwich method, and comprises magnetic beads coated with capture antibodies, a CEA standard, biotinylated detection antibodies, an SBG solution, a fluorogenic substrate solution and a sample diluent. The sensitivity of the Simoa kit reaches 0.1pg/mL, the sample loading amount only needs 10-20 mu L, the repeatability is good, the detection range is 1-20000pg/mL, and the CEA content in human body fluid can be accurately detected. |