| abstract |
The invention belongs to the technical field of brucella antibody detection, and particularly relates to a novel brucella antibody detection method. According to the invention, brucella outer membrane proteins are respectively marked by colloidal gold and latex microspheres, and form a 'cross-linked bridge' by capturing brucella antibody, on the basis of an agglutination experiment, the agglutination principle of brucella antibody is captured by the colloidal gold and the latex microspheres, and the result is tested by combining a spectrophotometer method, so that the detection sensitivity is greatly improved, the result can be obtained in 3-5 minutes, the sensitivity is high, the interpretation is objective, the operation is simple, and the detection and the use of basic personnel are facilitated. |