| abstract |
The invention provides a sample AFAI-DESI detection method, which comprises the following steps: staining a sample obtained after the tissue sample is frozen and sliced, and detecting by adopting AFAI-DESI, wherein the horizontal displacement rate is 0.03-0.05mm/s, the vertical displacement distance is 0.01-0.03mm, the atomized gas flow is 0.65-0.75MPa, the flow rate of the atomized solvent is 5.5-6.5 muL/min, the extending length of the spray needle is 0.45-0.55mm, and the included angle between the DESI spray needle and the glass slide is 45-55 degrees. The invention further provides the use of the above method for increasing spatial resolution. The sample AFAI-DESI detection method and the application thereof provided by the invention improve the spatial resolution of the technology to 20 μm, and simultaneously ensure the detection sensitivity and coverage of metabolites. |