http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113584127-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3b736d3c3724aa7c0c6aadb6d2e870b5 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 |
| filingDate | 2021-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8b85fa5dc1d959ec00528ef96d67b101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0aa638aa9eaf34e47699b50c400b8ecb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_57dc120e1065c23d1a906a13ecaefa46 |
| publicationDate | 2021-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113584127-A |
| titleOfInvention | Rapid screening method of gene editing single cells |
| abstract | The invention discloses a rapid screening method of gene editing single cells, which comprises the steps of carrying out amplification culture on a single cell in a 96-hole cell culture plate, taking out a part of cell suspension and placing the cell suspension in a 96-hole PCR plate; sealing, centrifuging, standing and centrifuging the 96-hole PCR plate, and absorbing the supernatant by using a 96-hole liquid absorption plate; adding 1xTE buffer solution with the pH value of 8.0 into the 96-hole PCR plate from which the supernatant is removed, sealing the 96-hole PCR plate, treating at 95 ℃ for 8-12 min, performing centrifugal treatment, and collecting the supernatant to obtain cell genome DNA; amplifying a gene segment containing a gene editing site by using cell genome DNA as a template, sequencing, and confirming a single cell edited by the gene according to a sequencing result; according to the method, the cells in the 96-well culture plate correspond to the 96-well PCR plate one by one, and then the cells are directly subjected to PCR amplification after DNA extraction, so that the method is simple to operate, time-saving and labor-saving, and the problem of manual plate number coding errors is avoided. |
| priorityDate | 2021-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 46.