http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113583910-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2b4c0b4bdf7052aa43dc78911f82ac63 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-066 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-06 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 |
| filingDate | 2021-08-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8c0f356fc9964f5bc5dc19ee1a231358 |
| publicationDate | 2021-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113583910-A |
| titleOfInvention | Preparation of Cell Extract from Large Intestine by Fermentation |
| abstract | The invention provides a cell extract prepared by a fermentation method for the large intestine, and relates to the technical field of cell extracts. The cell extract of the large intestine is prepared by the fermentation method, including cell fermentation and cell extraction. The cell fermentation includes the following steps: S1.1. Inoculate 10 liters of sterilized YTPG medium and 100 ml of fresh E. coli overnight culture into a fermenter; S1.2, culturing cells at 37°C with enhanced ventilation and agitation; S1.3, monitoring cell growth by continuously measuring OD600 (OD600 refers to the absorbance of a certain solution at a wavelength of 600 nm); S1.4, start cooling the cell culture medium when the cell growth reaches the middle stage, that is, when the OD600 is about 3-5. The use of scale-up preparations reduces the effect of this variable factor on cell-free expression, while also improving the reproducibility of experiments and the control of other optimized conditions. |
| priorityDate | 2021-08-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 77.