| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6fbb91e3f8cdf858527814216aa11142 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-622 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-543
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-26
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-74
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-005
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-74
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-26 |
| filingDate |
2021-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7247aa6c5e5af756e03a3483bf38b039
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b58633ebf52738f91048a38bd7906db5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_51c1ff19133326e0803737b036b0304c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a734a45cd5c4757a994b7042bc236467
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f0ed5e8232272b67f7003577c38ec417 |
| publicationDate |
2021-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-113583126-A |
| titleOfInvention |
Antiprogesterone scFv based on natural chicken-derived phage library and its preparation method and application |
| abstract |
The present invention is based on a natural chicken-derived phage library against progesterone scFv, a preparation method and application thereof, and aims to provide a method for screening progesterone by using a chicken-derived scFv natural phage display antibody library to obtain a single-chain antibody; Antigen (HT-OVA/HT-BSA) cross-coating, progesterone small molecule competitive elution strategy to obtain single-chain antibody with clear sequence; single-chain antibody with known sequence was transformed into prokaryotic cells by heat shock method for stable expression; The antibody has good stability, high expression level and high purity, which provides a fast and reliable preparation method for the acquisition and production of the antibody. A competitive enzyme-linked immunosorbent assay method was established for anti-progesterone single-chain antibody. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115975028-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115975028-B |
| priorityDate |
2021-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |