| abstract |
The invention provides a 5-11 double-ring sesterterpene skeleton compound and a preparation method thereof, wherein the structural formula of Nigtetraene is shown as follows, the amino acid sequence of synthetase NnNS is shown as SEQ ID NO.3, a synthetic gene is obtained by cloning from a CS12199 strain (Nectria. nigrescens 12199) genome, and a polynucleotide sequence is shown as SEQ ID NO. 1. The NnNS protein has the functions of catalyzing the chain length extension and structural cyclization of a substrate, and can assist the synthesis of the mother nucleus of the Nigtetraene compound. The invention is 5-11 bicyclicThe biosynthesis of the sesquiterpene compound provides a new resource, and a choice is provided for the synthesis of the compound types. |