| abstract |
The invention provides a double-target mRNA vaccine, which comprises a liposome mixture and double-target mRNA; the liposome mixture comprises DLin-MC3-DMA, DSPC, cholesterol and PEG200-DMG, and the molar ratio is 50:10:37.5: 2.5; the double-target mRNA is obtained by transcription of linearized recombinant plasmids; the recombinant plasmid contains a target gene segment; the target gene fragment is obtained by sequentially connecting the following modules in series: 5 '-UTR nucleic acid artificial sequence, P53 nucleic acid artificial sequence, Linker nucleic acid artificial sequence, Kras nucleic acid artificial sequence, MITD nucleic acid artificial sequence, and 3' -UTR nucleic acid artificial sequence; the invention also provides a preparation method of the vaccine; the mRNA vaccine prepared by the invention can block the migration of mRNA, protect the mRNA from being degraded and improve the delivery efficiency of the mRNA. |