| Predicate |
Object |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-998
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-999
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-91
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-31
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-30
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-166
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-12 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0609
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-061
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6888
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0604
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6879 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6888
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6879
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-076
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-075
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-073 |
| filingDate |
2021-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2021-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate |
2021-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-113564239-B |
| titleOfInvention |
Method for sex determination of early embryo of cow |
| abstract |
The invention relates to a method for sex determination of early-stage bovine embryos, which comprises the following steps: fertilizing the oocyte and the sperm in vitro to obtain a morula; cutting the morula in the cutting liquid drop by using a cutting knife to obtain an embryo tissue containing 10-12 cells as a sample for sex identification; preparing a sample by using a sex determination kit by a loop-mediated isothermal amplification method and preparing a Master Mix; after the samples are mixed, the reaction tube is placed in a loop-mediated isothermal amplification real-time turbidity detection system for reaction, and the samples are determined to be male, female and one of three situations which need to be redone indefinitely according to results. The method of the present invention exhibits excellent technical effects as described in the specification. |
| priorityDate |
2021-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |