http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113564108-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1c3535e924700de990503e8094eb00e0 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-84 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0663 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775 |
| filingDate | 2021-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_47552db47f18de36ca904d796492473e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9552beb6302dc787df7975a24b2e0fab http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eaa816883f061ee03744b4574a62f4d3 |
| publicationDate | 2021-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113564108-A |
| titleOfInvention | A method for in vitro expansion and culture of bone marrow mesenchymal stem cells |
| abstract | The invention discloses a bone marrow mesenchymal stem cell and a method for expanding and culturing the same in vitro. The method comprises: adding bone marrow cells to an erythrocyte lysis buffer and mixing, and then centrifuging to separate solid-liquid to obtain a solid; wherein, the The volume ratio of the bone marrow cells to the red blood cell lysis buffer is 1: (4.5-5.5); the solid is added to the primary culture medium for primary culture to obtain a culture; the primary culture medium is added to the The subculture is replaced with serum-free medium for subculture, and when the cells grow to 55-70% of the area of the cell culture flask, wash, digest and terminate the digestion, and collect the digested cells to be the P0 generation cells; The passaged cells are passaged and expanded to obtain bone marrow mesenchymal stem cells. The method can improve cell viability and cell purity, and increase cell proliferation rate. |
| priorityDate | 2021-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 19.