Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_37e3c09ac74b5fe31e0a912e4244f16e |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-96477 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-573 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54326 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-57488 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-532 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-57446 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-533 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-532 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-533 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-573 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-574 |
filingDate |
2021-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_14e6de3a07890fd8c1c0a17bcc2ea870 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c9d7f9cc21709b49bb5f8e9388c55de0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6b0dba2c65a2a8315988ac607578bfb4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c7d8f39a8acd98fc78373c31e141bd58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_71ce21618819e7f809599888f169a801 |
publicationDate |
2021-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CN-113552354-A |
titleOfInvention |
Pepsinogen multiplex detection method and detection kit |
abstract |
The invention discloses a pepsinogen multiplex detection method and a detection kit, comprising the following steps: 1) synthesizing two magnetic coding microspheres with different fluorescence intensities; 2) coupling a PG I capture antibody and a PG II capture antibody on the magnetic coding microspheres; 3) preparing a PG I detection antibody and a PG II detection antibody marked with the same biotin; 4) immune reaction: 5) detecting the magnetic coding microsphere solution obtained in the step 4) by using a flow cytometer, and respectively calculating the concentrations of pepsinogen PG I and pepsinogen PG II in the sample to be detected according to the fluorescence intensity of the two magnetic coding microspheres. The detection method provided by the invention can simultaneously detect pepsinogen PG I and PG II in a patient blood sample, and two objects to be detected can simultaneously complete analysis, so that the operation steps are reduced, the detection errors caused by different reagents in an experiment are avoided, the detection time is shortened, and the efficiency and the detection accuracy can be greatly improved. |
priorityDate |
2021-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |