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filingDate 2021-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_14e6de3a07890fd8c1c0a17bcc2ea870
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publicationDate 2021-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-113552354-A
titleOfInvention Pepsinogen multiplex detection method and detection kit
abstract The invention discloses a pepsinogen multiplex detection method and a detection kit, comprising the following steps: 1) synthesizing two magnetic coding microspheres with different fluorescence intensities; 2) coupling a PG I capture antibody and a PG II capture antibody on the magnetic coding microspheres; 3) preparing a PG I detection antibody and a PG II detection antibody marked with the same biotin; 4) immune reaction: 5) detecting the magnetic coding microsphere solution obtained in the step 4) by using a flow cytometer, and respectively calculating the concentrations of pepsinogen PG I and pepsinogen PG II in the sample to be detected according to the fluorescence intensity of the two magnetic coding microspheres. The detection method provided by the invention can simultaneously detect pepsinogen PG I and PG II in a patient blood sample, and two objects to be detected can simultaneously complete analysis, so that the operation steps are reduced, the detection errors caused by different reagents in an experiment are avoided, the detection time is shortened, and the efficiency and the detection accuracy can be greatly improved.
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type http://data.epo.org/linked-data/def/patent/Publication

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