| abstract |
The invention discloses a method for researching influence of chicken EZH2 gene on neoplastic diseases, comprising S1, purifying chicken EZH2 prokaryotic protein to obtain an EZH2 protein sample; s2, co-culturing the purified chicken EZH2 prokaryotic expression protein and an MSB1 cell, and determining the influence of the EZH2 prokaryotic expression protein on the MSB1 cell proliferation; s3, extracting total RNA of cells at different time, detecting and analyzing the expression level of the MDV related gene, and determining the influence of the EZH2 prokaryotic expression protein on the expression level of the MDV related gene; experiments prove that the chicken EZH2 protein can influence the proliferation of MSB1 cells and the expression of MDV Meq and gB genes in the cells, and lays a foundation for further researching the action mechanism of the chicken EZH2 in MDV infection-induced tumors. |