| abstract |
The present invention provides a method for reducing antibody galactosylation, and relates to the field of biotechnology. Including: using galactose analogs in the process of culturing cells expressing the antibody, the galactose analogs are selected from compounds of formula (1) or biologically acceptable salts thereof, wherein R 1 , R 2 , R 4 is independently selected from one of halogen or -OH; R 3 is selected from -OH or -OAc. The method of the invention adds a certain concentration of galactose analogs in the process of cell culture, which can effectively and stably regulate galactosylation, so that the ratio of each glycoform is controlled near the target level. |