| abstract |
The present invention relates to the technical field of medicine, and in particular to a nucleic acid construct comprising a beta globin gene, the beta globin gene comprising exons, introns and cis-acting elements, the cis- The acting elements include one or more of WPRE, SV40 polyadenylation signal or SV40 ori and truncated site control regions. The nucleic acid construct virus packaging efficiency of the invention is significantly enhanced, the lentiviral vector has stronger integration ability, the recombinant lentiviral gene therapy vector has high production efficiency, low production cost, wide application range, and can be used for the production and preparation of various forms of hereditary anemia Gene therapy lentiviral vector. |