http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113549636-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0d653493ab5b577e9282e70dab026392 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-22 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01017 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2462 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-56 |
| filingDate | 2021-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9a91321281157ac4bd4068fe4283a710 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d0b46ce0056cc5296d1f0a5398f6d920 |
| publicationDate | 2021-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113549636-A |
| titleOfInvention | A codon-optimized zebrafish g-type lysozyme-1 gene and its recombinant expression protein |
| abstract | The invention discloses a codon-optimized zebrafish g-type lysozyme-1 gene and a recombinant expression protein thereof. There are three lysozyme genes in zebrafish, namely c-type lysozyme, g1-type lysozyme and g2-type lysozyme. Described zebrafish g-type lysozyme-1 gene is obtained through optimization, and is obtained by means of whole gene synthesis after codon optimization, its nucleotide sequence is as shown in SEQ ID NO.2, and its encoded amino acid sequence is as follows: shown in SEQ ID NO.3. The gene was connected with the vector pET-28a(+) to obtain the recombinant plasmid 28a-Lys-g1, which was further transformed into E. coli BL21 (DE3) competent cells. After induction expression, purification and SDS-PAGE verification, after renaturation, high Zebrafish g-type lysozyme-1 recombinant protein with purity and high bacteriolytic activity. |
| priorityDate | 2021-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 187.