http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113528598-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_82527fd92ad2f36998c602e6dacf6c41 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-305 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-30 |
| filingDate | 2021-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7464e206ba4d04871e10e80328937bfc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a87a21804ea49c546be6dd6b4347e589 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_caf9ff254cbc3853cfd70d26fe9a31e3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9eb836cf7c73ddb072608d3f25b7d5fc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3c641397c28dbdd926ce85b875713246 |
| publicationDate | 2021-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113528598-A |
| titleOfInvention | Pretreatment method of RNA enzymolysis liquid |
| abstract | The invention discloses a pretreatment method of RNA enzymolysis liquid, which comprises the following steps: RNA lysis solution: the total volume is 6L, wherein the RNA dosage is 2 percent, the solid enzyme dosage is 5 percent, and the zinc sulfate dosage is 0.5 percent; RNA reaction: taking two groups of 3L RNA dissolving solutions respectively, then carrying out RNA reaction respectively at the reaction temperature of 70-72 ℃ for 3h, then sampling at the reaction time of 2.5h and 3h, and carrying out HPLC (high performance liquid chromatography) detection on the reaction conversion rate; RNA loading: respectively loading the ultrafiltrate and the RNA reaction solution, judging that the sample loading is leaked, and recording the sample loading amount and the sample loading volume, wherein the leakage concentration C is more than or equal to 0.4g/L, and the ratio of the concentration of the sample loading solution to the concentration of the leaked solution is more than or equal to 1. According to the invention, through a series of pretreatment combinations such as plate-frame filtration, microfiltration, ultrafiltration and cation resin, impurities such as macromolecular protein, particle impurities, pigments, soluble protein, calcium, magnesium and zinc ions contained in raw materials such as RNA and enzyme can be removed, the impurities adsorbed on the anion exchange resin are reduced, the anion exchange resin is effectively protected, and the service life of the anion exchange resin is prolonged. |
| priorityDate | 2021-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 39.