http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113528566-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02E50-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-84 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-67 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 |
| filingDate | 2021-06-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113528566-B |
| titleOfInvention | A yeast recombinant expression vector and its construction method and application |
| abstract | The invention relates to a yeast recombinant expression vector and its construction method and application. The yeast recombinant expression vector contains an expression frame, and the expression frame sequentially includes a gene of a target protein to be expressed located in the same cistron, and a protease cleavage site. The coding sequence of the point and the antibiotic resistance gene; the protease cleavage site can be specifically recognized and cut by the protease in yeast. The invention also provides the construction method of the yeast recombinant expression vector and its application in screening the recombinant yeast highly expressing the target protein. By applying the technical scheme of the present invention, the recombinant yeast with high expression of the target protein can be screened quickly and with high throughput. Compared with the prior art, the rescreening time is significantly reduced, the false positive rate is reduced, and the screening efficiency is improved. . |
| priorityDate | 2021-06-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 38.