| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_53b4d53983adbf56dd0e7d0bf96f8ce5 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-54
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-75
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C14C1-065 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C14C1-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-75
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-57
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-54 |
| filingDate |
2020-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_66bb74276a654a052ffb79ae0f95065d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_64940d09f339657c514f06a68679b236
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_99c7f87a49ff22fbe730e9b4c0f86bfa |
| publicationDate |
2021-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-113528556-A |
| titleOfInvention |
Heterologous expression of a keratinase and its application in sheepskin depilation |
| abstract |
The invention discloses heterologous expression and characterization of a Bacillus subtilis keratinase and its application in sheepskin depilation, and belongs to the field of biotechnology. The full length of the keratinase gene of the present invention is obtained by mining the full gene data of Bacillus sp. LCB12, the full length of the gene is 1140bp, encoding 379 amino acids, and the gene is amplified in vitro by PCR. The present invention realizes the heterologous expression, purification and characterization of keratinase VT104K gene in Bacillus subtilis SCK6 for the first time, and applies the recombinant keratinase to goat skin depilation. The keratinase has good enzymatic properties and excellent depilation performance, and has broad application prospects in the tanning process. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115323080-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112175977-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112175977-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115323080-A |
| priorityDate |
2020-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |