| abstract |
The invention discloses a NtQPT2 gene mutant that reduces the nicotine content of tobacco leaves, which is characterized in that: the nucleotide sequence of the NtQPT2 gene mutant that reduces the nicotine content of tobacco leaves is shown in SEQ ID NO: 3, which is the same as the nucleotide sequence. Compared with the NtQPT2 gene shown in SEQ ID NO: 1, the NtQPT2 gene mutant that reduces the nicotine content of tobacco leaves has a mutation of c.229-230insA, which changes the protein sequence encoded by the NtQPT2 gene after the mutation site, and forms Truncated mutant amino acid. Through systematic research, the present invention proposes for the first time a NtQPT2 gene mutant that reduces the nicotine content of tobacco leaves. Tobacco plants obtained from the NtQPT2 gene mutant that reduces the nicotine content of tobacco leaves proposed by the present invention have 88% lower nicotine content than the control. %, the nicotine content of tobacco leaves was significantly reduced. |