http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113528479-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3c96af599b7d29b6715a68a0b4c224a1 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-77 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1044 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y203-02013 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-15 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-77 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 |
| filingDate | 2020-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_094ebf7fcb560d119e9e9f1ab6508099 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3fdbb52d3812dd555b2934a2ebdebf3a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bc027799b5a097d6e5e4d8ee72a1fb52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_789dda7e7fc7fb53001fa968d4829b10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c38f6df60421ed642f0cf15bd68a81ff |
| publicationDate | 2021-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113528479-A |
| titleOfInvention | A kind of efficient preparation method of transglutaminase and its special engineering bacteria |
| abstract | The invention discloses an efficient preparation method of transglutaminase and a special engineering bacteria thereof. The present invention provides a protein, which is the protein shown in SEQ ID NO: 2 of the sequence listing. The present invention also protects the recombinant microorganism obtained by introducing the DNA molecule expressing the protein into Corynebacterium glutamicum. The recombinant microorganism can be used to produce transglutaminase. The present invention expresses the MTG derived from Streptomyces maoyuan under the T7 promoter, and inserts the ΔICM intein between the pro region and the mature region of the MTG to successfully realize the high-efficiency expression of MTG in Corynebacterium glutamicum, After the bacterial cells obtained by fermentation are broken by ultrasonic, the MTG with biological activity can be obtained without protease treatment or other changes of conditions. The invention can be used for preparing transglutaminase, and further applied to the fields of tissue engineering, textile and leather processing and the like, and has application and popularization value. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115074304-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115074304-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115093470-A |
| priorityDate | 2020-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 363.