http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113528478-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-77 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y203-02013 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1044 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-77 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2020-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2023-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2023-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113528478-B |
| titleOfInvention | A method for efficiently producing transglutaminase and its special engineering bacteria |
| abstract | The invention discloses a method for efficiently producing transglutaminase and special engineering bacteria thereof. The present invention provides a protein, which is the protein shown in Sequence 2 of the Sequence Listing. The invention also protects the recombinant microorganism obtained by introducing the DNA molecule expressing the protein into Corynebacterium glutamicum. The recombinant microorganism can be used to prepare transglutaminase. In the present invention, the MTG derived from Streptomyces Maoyuan is expressed under the T7 promoter, and the intein ssp-dnaB is inserted between the pro region and the mature region of MTG to successfully realize the high efficiency of MTG in Corynebacterium glutamicum For expression, MTG with biological activity can be obtained without protease treatment or other condition changes after the bacterial cells obtained from fermentation are ultrasonically disrupted. The invention can be used to prepare transglutaminase, and then applied to the fields of tissue engineering, textile and leather processing, etc., and has the value of application and popularization. |
| priorityDate | 2020-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 368.