http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113527504-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_04361643f217360425d07cf6f6526c4c |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-765 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K17-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-577 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K17-08 |
| filingDate | 2021-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aa7d695297c47dff69e7ddef164f44f9 |
| publicationDate | 2021-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113527504-A |
| titleOfInvention | A Bioorthogonal Reaction-Based Directed Antibody Immobilization Method |
| abstract | The invention discloses a bio-orthogonal reaction-based directional antibody immobilization method, comprising the following steps: introducing an enzyme with bio-orthogonal reaction activity as a label, and linking with an antibody to be immobilized by fusion to obtain an antibody with a fusion enzyme label . The high-purity fusion enzyme-tagged antibody is further reacted with the alkyl halide dehalogenase substrate 6-chlorohexane to form a covalent bond to prepare a surface-functionalized carrier surface. The antibody solution with the alkyl halide dehalogenase label was incubated with the surface of the solid support modified with the alkyl halide dehalogenase substrate, and the biological normalization of the alkyl halide dehalogenase with the substrate 6-chlorohexane was carried out. The cross-reaction achieves one-step covalent immobilization of the antibody-functionalized solid support surface. The high specificity of the bioorthogonal reaction is used to immobilize the target antibody through covalent bonds, so as to realize the immobilization direction and irreversibility, maintain high stability in different chemical and physical environments, and can be applied to the detection of complex biological systems. and sample affinity purification. |
| priorityDate | 2021-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.