| abstract |
The invention discloses a tulathromycin hapten, an artificial antigen and an antibody, a preparation method and application thereof, the tulathromycin hapten provided by the invention furthest reserves the characteristic structure of tulathromycin, and introduces maleimide group,the immunogenicity of the tulathromycin hapten is obviously enhanced; the tulathromycin artificial antigen obtained by coupling tulathromycin hapten and carrier protein is used for immunizing animals, so that the tulathromycin artificial antigen is more favorable for stimulating the immune response of the animals to generate antibodies with stronger specificity and higher sensitivity, and the IC of the tulathromycin antibodies to the tulathromycin is detected by an indirect competitive enzyme-linked immunosorbent assay 50 Is 0.3 mu g/L, has low cross reaction rate with other macrolide antibiotics, and provides a foundation for the subsequent establishment of various immunoassay methods of the tulathromycin. |