http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113512573-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_62104d7901968b2f88e500fc9ad5345b |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-998 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-999 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-04 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-5412 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-5431 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-5415 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-475 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0663 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-475 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-535 |
| filingDate | 2021-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_86fcf4c84028fd1a2a86f946c11a1557 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b424f802c3069be3daddc42c1981ea18 |
| publicationDate | 2021-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113512573-A |
| titleOfInvention | Method for promoting mesenchymal stem cells to secrete cytokines |
| abstract | The invention discloses a method for promoting mesenchymal stem cells to secrete cell factors, which comprises the following steps: (1) separating bone marrow mesenchymal stem cells, carrying out primary culture, and digesting with 0.25% pancreatin after the cell fusion degree reaches 80-90%; (2) and (2) after the cells digested in the step (1) are resuspended by using a culture medium, the cells are inoculated into a culture bottle coated by hydroxypropyl cellulose and poloxamer 188, half amount of liquid change is carried out after 24h of culture, the culture medium is supplemented once a day in the continuous culture process, paeonol, metallothionein-3 and rapamycin are added into the supplemented culture medium every time, continuous culture is carried out for 3 days, and the culture solution is collected. The cells after primary culture are cultured by adopting a culture bottle coated by hydroxypropyl cellulose and poloxamer 188, and a culture medium containing paeonol, zinc metallothionein and rapamycin is supplemented in time in the culture process, so that the secretion of cell factors by the mesenchymal stem cells is remarkably promoted, and the collection and application of the mesenchymal stem cell factors are facilitated. |
| priorityDate | 2021-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 187.