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filingDate 2021-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2867164194f91c501e54a52fc2ec63ce
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publicationDate 2021-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-113512560-A
titleOfInvention Method and application of improving the resistance of Lentinus edodes to Trichoderma dark green by overexpressing Lentinus edodes TLP gene
abstract The invention belongs to the field of biotechnology, and in particular relates to a method and application for improving the resistance of Lentinus edodes to Trichoderma dark green by overexpressing the Lentinus edodes TLP gene. In the present invention, the strong anti-strain Y3334 cDNA is used as a template to amplify the full-length fragment of the LeTLP1 gene, and the linearized vector pCAMBIA1300-g, the Legpd promoter and the full-length fragment of the LeTLP1 gene are connected by recombinase to construct a Lentinus edodes LeTLP1 gene overexpression vector to obtain overexpression Transformants, studied the effect of overexpression of LeTLP1 gene on the resistance of Trichoderma dark green mushrooms, the results found that overexpression of LeTLP1 gene can enhance the resistance of weak resistance to T. Methods The resistance of Lentinus edodes strains to Trichoderma was enhanced.
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