| abstract |
The invention discloses a hybridoma cell line, whose deposit number is CGMCC No.18521. The invention also discloses the monoclonal antibody secreted and produced by the hybridoma cell line CGMCC No.18521. The invention also discloses the coding gene of the monoclonal antibody, the recombinant anti-pig-derived APN protein antibody protein expressed in vitro and the preparation method thereof, and the application of the antibody protein in the preparation of medicines or vaccines for preventing or treating intestinal diseases. The APN antibody obtained by the invention can specifically bind to the pig-derived APN protein, has strong affinity and good stability, and lays a foundation for further studying the function of APN and developing genetically engineered antibody drugs and new vaccines. |