| abstract |
The invention discloses a biomass-based encapsulation material and an encapsulation method for active protection of probiotics, including a nano-film for encapsulating probiotics; the nano-film includes two layers, and the first layer is a natural bio-based polymer It is formed in situ with metal ions on the surface of probiotics through covalent cross-linking or metal chelation; the second layer is formed by the interaction of biological enzymes and natural bio-based macromolecules; the nano-film formed in situ on the probiotic cell wall of the present invention , used for the encapsulation protection of probiotics; the nano-film is composed of natural biomass, with low cost and no toxic side effects; whether the encapsulated probiotics are cultivated in a high-concentration antibiotic or active free radical environment, their relative activity The relative activity of unprotected probiotics is below 30% or even completely non-viable. |