| abstract |
The present invention provides a method for preparing effective parts of cannabinoid from hemp seeds or hemp seed husks, which has the characteristics of simple process, easy amplification, high product yield and the like. The method comprises the following steps: 1) pulverizing the dried hemp seeds or hemp seed husks into coarse powder; 2) extracting the coarse powder with an aqueous ethanol solution, performing solid-liquid separation on the obtained extract, and separating the obtained supernatant Concentrate into a concentrated solution; 3) leave the concentrated solution to stand for precipitation, and invert the resulting precipitate with an aqueous ethanol solution to obtain a trans solution; 4) separate the trans solution obtained in step 3) by semi-preparative chromatography, and the separation of the semi-preparative chromatographic conditions Including: the preparation column is a C18 chromatographic column, with phosphoric acid aqueous solution and methanol as mobile phases, the detection wavelength is 255-257 nm, and the effluent corresponding to the characteristic peak of the chromatogram of cannabinamide A is collected; 5) After the effluent is combined, concentrated and dried The finished product of the effective part of cannabidiol is obtained. |