http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113493803-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8289 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8297 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8218 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8205 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-415 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-29 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-54 |
| filingDate | 2021-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113493803-B |
| titleOfInvention | Alfalfa CRISPR/Cas9 genome editing system and application thereof |
| abstract | The invention discloses an application of a CRISPR/Cas9 genome editing system in alfalfa gene editing, wherein the CRISPR/Cas9 genome editing system comprises an expression vector, the expression vector can comprise a Cas9 expression cassette, a sgRNA1 expression cassette and a sgRNA2 expression cassette, and the Cas9 expression cassette expresses Cas 9. The sgRNA1 expression cassette expresses a sgRNA named sgRNA1, and the sgRNA2 expression cassette expresses a sgRNA named sgRNA 2; the sgRNA1 expression cassette contains a promoter named MtU6-6promoter and a sgRNA1 gene driven by the MtU6-6promoter, and the sgRNA2 expression cassette contains a promoter named MtU6-5promoter and a sgRNA2 gene driven by the MtU6-5 promoter; the sgRNA1 and the sgRNA2 can be directed against the same target gene or different target genes of alfalfa. The invention designs double targets for Target genes according to the characteristics of alfalfa autotetraploid, constructs a gene editing binary vector p6401-Target, and obtains regenerated plants by agrobacterium-mediated transformation of alfalfa. The optimized genome editing system greatly improves the alfalfa gene editing efficiency, the plant gene editing efficiency can reach 100%, and the single target point editing efficiency can reach 96.9%. |
| priorityDate | 2021-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 50.