| abstract |
The invention discloses a goat infectious pleuropneumonia genetic engineering subunit vaccine and its preparation method and application. The vaccine comprises a first recombinant protein, a second recombinant protein and a pharmaceutically acceptable carrier, the first recombinant protein and the second recombinant protein respectively have the sequences shown in SEQ ID NO: 2 and SEQ ID NO: 6 . The vaccine provided by the invention has no toxicity, high safety and good immunogenicity, and can generate strong humoral immunity in sheep, and the antigens expressed by the first recombinant protein and the second recombinant protein have good crossover Protective effect, can simultaneously resist the attack of Mycoplasma capricolum subsp. capricolum, Mycoplasma capricolum subsp. capricae and Mycoplasma ovine pneumoniae, immunized sheep can resist virulent challenge, and can also use bioreactor large-scale serum-free suspension culture The preparation has the advantages of easy quality control, batch-to-batch stability, and low production cost. |