| abstract |
The present invention provides an explosive visualization biosensor based on yeast cell surface display technology, and a preparation method and application thereof. The explosive visualization biosensor contains an anchor protein encoding gene and a target gene dntA or dntB gene for explosive visualization. The present invention uses the anchor protein to anchor the target gene expression product to the surface of the host bacteria, and finally obtains the explosive visualization biosensor. The biosensor can specifically degrade 2,4-dinitrotoluene (2,4-DNT) into a red substance, 2-hydroxy-5-methylbenzoquinone, which is visible to the naked eye, and then judges whether there is a substance in the sample to be tested. Explosive molecules, and it has the maximum absorbance value at 420nm, the concentration of 2,4-DNT can be calculated according to the standard curve, its stability is very good, and the detection range is wide, the sensitivity is high, the method is simple, the cost is low, and the safety is strong, Therefore, it has broad application prospects. |