http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113466111-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7a026d8a70c752fdff41d1e7e64fd628 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2015-1006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2015-105 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6486 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N15-10 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-01 |
| filingDate | 2021-07-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_02ac4239a582628f9bb8446fec3d3690 |
| publicationDate | 2021-10-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113466111-A |
| titleOfInvention | Single cell analysis system and method and application |
| abstract | The invention discloses a unicellular analysis system combining optical tweezers and confocal up-conversion fluorescence imaging, which comprises a collimator, a climbing frame, a beam expanding system, a telescope system, an inverted fluorescence microscope, a high-numerical aperture objective lens, a cell culture special chamber, a confocal scanning imaging module coupled with a 980 nm near-infrared continuous laser, and a fluorescence signal collector based on an area array imaging device, and also discloses an analysis method, wherein the biomolecule to be detected in adherent cells is specifically marked by a biological sensing strategy, then the cells are digested and dispersed in a sample chamber, then single suspended cells are captured by the optical tweezers, the up-conversion fluorescence high-resolution imaging of the cells is realized at the same time, finally the physiological state of the cells is changed by a specific biochemical reagent, and the real-time change condition of the content of the substance to be detected in the cells is monitored, the method has the advantages of high imaging stability, high sensitivity, low background signal, strong anti-interference capability, small sample consumption and the like. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114264639-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114813706-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114264639-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114813706-B |
| priorityDate | 2021-07-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.