abstract |
The invention provides a covalent immobilized cell and a preparation method and application thereof, belonging to the field of bioengineering; in the present invention, a covalently immobilized cell is first prepared, and the covalently immobilized cell is made of Fe 3 O 4 @SiO 2 ‑NH 2 -alkyne is a carrier, and is constructed by connecting azido modified recombinant escherichia coli; then the covalent immobilized cells are applied to bioconversion of 1,3-dihydroxyacetone, and the reaction condition in the bioconversion process is mild, the specificity is strong, the environment is protected, and the utilization rate of the substrate is high. |